Science in Action: Omega-3 (a new test)

Two days ago I explained why the test I was using to measure my mental function many times/day had room for improvement. I wanted a new test much like the old test but with which my accuracy was higher.

I was more accurate with the simple arithmetic test (e.g., 3 + 6) than with the memory test I described two days ago. The crucial difference might have been the number of possible answers. The arithmetic test had 40-odd possible answers; the memory test had 2 (yes and no). Saul Sternberg did a reaction-time experiment in which the number of possible answers was varied from 2 to 8. I don’t know what the accuracy data were but the variance of the reaction times was lower with 8 possible answers even though reaction times were longer. A plausible explanation is that there was much more anticipation with 2 possible answers than with 8. Anticipation can cause errors.

The new test I am trying consists of typing how many letters from the set {A, B, C, D} are among a set of four letters chosen from a much larger set (most of the alphabet). The possible answers are “1″, “2″, “3″, and “4,” each equally likely. For example, I might see T B X A. The correct answer is “2″. I am using R (the programming language) to run this test so I type “2″ with one hand and hit Enter with the other as fast as possible.

Here are the results so far from the new test — the training phase.

mean RT

These values are taken from fit of a linear model; they are similar to means. As I gain experience with the test I am getting faster. The new test is slower than the old test (which is good — more mental processing).

Consistent with what Sternberg found, variation in reaction times is less with the new test than with the old test even though average reaction times are greater:

This graph shows the standard deviation of residuals from the fitted model. The units are reciprocal seconds (x 10) because I did a reciprocal transformation before fitting the model. The reciprocal transformation made the reaction times close to normally distributed.

Here is accuracy:

The new test feels easier than the old test, but so far there is little difference.

Overall it seems to be a step in the right direction. Reduction in variation of reaction times means more sensitive measurements.

The experiments I am planning are very simple: Test myself regularly (say, every half-hour), eat something. If the measurements are steady, it is very easy to see an effect. As far as I know, such experiments have never been done. One reason, I think, is that they require self-experimentation: It is no trouble for me to do the test (which takes 4 minutes) 100 times in a week and thereby reach a steady state. But to have someone else do the test 100 times as preparation — especially if the test were done in a lab — would be very difficult.

Science in Action: Omega-3 (follow-up of surprise)

During a trip to Los Angeles a few weeks ago, I noticed that my scores on several mental tests were better all of a sudden. The apparent cause was that I had taken flaxseed oil at an unusual time. Normally I took it about 10 hours before the tests; in this case I had taken it about 4 hours before.

Does flaxseed oil have a short-lived effect on brain function? When I got home I tried to find out. Rather than doing a set of four tests once per day I switched to one test many times per day (e.g., 10 times). This would allow detection of short-lived ups and downs in my mental function.

The test I used required nothing but my laptop. I usually have my laptop with me so such a test is much easier to do throughout the day than a task that requires other equipment. The test consisted of four blocks of 50 trials each. For each block I memorized a new set of three digits (e.g., 0 1 7). On each trial I saw 1, 3, or 5 digits and pressed a keyboard key as quickly as possible to indicate if any of the memorized digits is in the displayed set. For example, if the memory set was 0 1 7 and the display set was 3 2 8 the correct answer was “no” (which I indicated by pressing “4″).

The trials were packed together as closely as possible: As soon as I answered, the next set appeared. It took about 3 minutes to do 200 trials.

I did frequent measurements for four or five days. They appeared to confirm what the Los Angeles measurements suggested: Flaxseed oil did have a short-term effect. But two things muddied the water:

1. Baseline measurements were not always as steady as I would like. There were ups and downs that seemed too large to be random variation. The curious and exciting thing was that these ups and downs usually had a possible explanation — something had changed. For example, the measurements would be X1, X2, X3, Y. X1, X2, and X3 are close; Y is quite different. Between X3 and Y I had eaten a meal.

2. The task was difficult. I was about 88% correct and it was hard to do better. With any reaction-time task there is a speed-accuracy tradeoff: If you are slower, you can be more accurate. In this particular case this is a problem because it is an added source of variation and may reduce reaction-time differences: Rather than becoming slower, I become less accurate (or rather than becoming faster I become more accurate).

Problem #1 is easy (if slightly unpleasant) to solve: Keep the situation more constant. Eat less during the measurement period, etc.

To reduce Problem #2 I am learning a new task. I will go into detail tomorrow.

Omega-3: I Can See For Myself

“The flax seed oil scam” by a herbalist named Henriette says bad things about flaxseed oil. One is about (lack of) conversion of ALA (the short-chain omega-3 in flaxseed oil) to EPA and DHA (the long-chain omega-3s found in fish oil and presumably active in the brain):

The scam is in flax seed oil folks trying to maintain that we can convert ALA into EPA and DHA in anything like relevant amounts.

We can’t. We convert at most 10 %, but usually less than half that.

Which is “fairly common knowledge among nutritionists,” says Henriette. She quotes the abstracts of two scientific papers to support this point. The other criticism is that flaxseed oil goes bad quickly:

I dislike flax seed oil for another reason as well: it oxidizes (goes rancid) pretty much the minute it’s pressed, and unless it’s been refrigerated ALL the way from press to consumer, it’s ALWAYS rancid.

After I read this, I realized I was in an unusual position. When it comes to flaxseed oil, I don’t have to take anyone’s word for it. I have been able to measure the benefits by myself on myself. Apparently the conversion ratio, whatever it is, is high enough; and the suppliers of my flaxseed oil (I have used Spectrum Organic, Barlean’s, and the Whole Foods house brand) have solved the oxidation problem.

With almost every other nutrient, my knowledge is far less certain. Sure, I need some Vitamin C, but how much is best? Too much may cause cancer. I’ll probably never know the best amount for the average person, much less the best amount for myself.

Omega-3 and Dental Health: Surgery Commuted

I started writing a follow-up to this Marginal Revolution post by Tyler Cowen before I knew of its existence:

January [2007] entodontist [= gum specialist]: “You’ll need surgery either right now, or within a few months. We cut open the gum, clean out the inflammation, and sew your mouth right back up. Only sometimes do we have to eliminate the tooth.”

July 5 [2007] entodontic surgeon, 10:31 a.m.: “We can cancel this morning’s surgery, it seems OK for now, just keep an eye on it.”

In June, Tyler posted about the benefits he derived from flaxseed oil (2 Tablespoons/day): “Very good for my heart, my brain, and my gums.” I asked him what was better. “Much better gums, for sure,” he replied. “The rest is harder to measure.” On July 4 I got around to asking for details. Tyler said that he had had bad gums for most of his life and that he noticed they were much better within a week or two of starting the flaxseed oil. He added

I have crooked wisdom teeth, never wore braces, and my mouth naturally produces lots of plaque. Put all together that means a significant problem with gum disease. I get cleanings every three months or so but still my gums have been much worse than average.

I too have crooked teeth and more plaque than average and I too found that flaxseed oil improved my gums; my dentist was the first to notice.

A recent experiment about omega-3 and dental health. A 1997 experiment. An amazing bowling video.

Science in Action: Omega-3 (what the results mean)

How do I interpret the results so far of my omega-3 self-experimentation? I’m going to skip the obvious implications (I should do more experiments, I should take omega-3, . . . ) and jump to the less obvious ones:

1. Omega-6 may make things worse. The difference between flaxseed oil and olive oil was larger than the difference between flaxseed oil and nothing, implying that olive oil is worse than nothing. Perhaps this is because olive oil is relatively high in omega-6, which displaces omega-3. The Israeli Paradox points in the same anti-omega-6 direction as do lab experiments that suggest omega-6 fats are pro-inflammatory.

2. I should study other fats. My experiments don’t just imply that omega-3 fats have a big effect on brain function, they imply that fats in general have big effects — and that these effects can be easily measured (which is the interesting part).

3. Health providers should pay far more attention to brain function — to “brain health.” Improvements in balance led me to treatments that improved my performance on memory tests. Not surprising, since the whole brain is made of the same stuff (neurons, glial cells, etc.), but it implies that with easy to administer tests you could catch a wide range of brain problems long before they cause serious difficulties, such as dementia, Alzheimer’s, and injury-causing falls. Note that no doctor ever orders tests similar to those I have used. Yet my tests eventually revealed that I was suffering from what might be called omega-3 deficiency. One well-accepted test of mental function is the Mini-Mental State Exam. It consists of such questions as “What month of the year is this?”. By the standards of experimental psychology, it is incredibly crude. Experimental psychologists have a lot to teach the health community about how to measure brain function.

Omega-3 and Cancer

A just-published article in the American Journal of Epidemiology reports a very clear negative correlation between colorectal cancer and omega-3 consumption. It describes the results of a case-control study done in Scotland from 1999 to 2006. The investigators hoped to recruit all cases of colorectal cancer coming for surgery in Scotland; they managed to recruit about half of them and ended up with about 1500 “cases.” Each case was paired with a healthy control matched for age, sex, and residence. Then they compared the diets of the two groups. This is the approach that first linked smoking and lung cancer. Lung-cancer patients were more likely to smoke than other types of patients.

In the Scotland study, there was no correlation between cancer and overall fat consumption, but there was a very clear correlation with omega-3 fat consumption: more omega-3, less cancer. There was no correlation with omega-6 fat consumption. The conclusions remained the same after they combined their results with four previous similar studies.

Science in Action: Omega-3 (data from my mom)

My mother tried drinking flaxseed oil. She measured her balance by standing on one leg; the measure was how long she could do that. She did ten of these measurements per day.

Here is what happened:

effect of flaxseed oil on balance

When she started taking the flaxseed oil, her balance suddenly started to improve.

Are injury-causing falls “the new scurvy,” I wondered — that is, caused by an easily-preventable nutrient deficiency? These results support that idea. However, I can’t explain the decline in balance during the pre-flaxseed baseline period. Perhaps she had eaten food high in omega-3 and the effects were wearing off.

Here are precautions about flaxseed oil. If you are 70 years old or older and would like to find out if flaxseed oil improves your balance, please contact me.

Directory of my omega-3 research.

Science in Action: Omega-3 (a surprise!)

I have always stopped self-experimenting when I travel because so much changes. Surely I will sleep differently, etc., far from home. However, it is not so obvious my arithmetic speed (how fast I do arithmetic problems such as 6 + 3) will change. I am measuring arithmetic speed as part of my study of omega-3 (directory).

I recently spent a week in Los Angeles. For the first time I continued self-experimentation while traveling. When I arrived I bought a bottle of flaxseed oil. I continued to take 4 T/day and did the same mental-function tests I do at home: arithmetic, memory-scanning, and balance. I have described these tests in other posts.

My balance was much worse in Los Angeles, apparently because what I see during the test changed (because the floor and other surroundings are different). I hadn’t realized how much that mattered. My arithmetic and memory-scanning results were roughly the same as the results at home — that is, until the last day. This graph shows arithmetic speeds:

arithmetic speed

This graph shows memory-scanning results:

memory scanning speeds

The sudden improvement on the last day — also clear in the balance test — was a big surprise. It was too large to be due to practice, nor could it be due to being in LA — the previous 5 measurements were also in LA. It did, however, have a ready explanation: The previous night I had gotten back late and had forgotten to take the oil. So instead of taking 4 T at 11 pm I took it at 7 am. I did the tests at about noon. Instead of 8 or 9 hours between oil ingestion and test, in this case the difference was 5 hours.

If this explanation is correct, there is a short-lived effect of flaxseed oil on brain function — present 5 hours after ingestion but absent or weaker 8 hours later. Which, as a scientist, makes me say “Wow!” If this effect exists, it’s a new tool, the most precious and powerful thing in science. I can use it to compare amounts of flaxseed oil, oils (e.g., fish oil), and foods (e.g., salmon).

My current way of measuring omega-3 effects requires one/day tests repeated for weeks. When I reduced the amount of flaxseed oil I was taking from 4 T/day to nothing, it took more than a day with the lower dose before performance even went down, and many more days before performance stabilized. This meant that experiments had to last several weeks. If the new effect exists, it will allow much faster experiments.

Shopping Notes

1. At a Vietnamese take-out place near Berkeley I got a can of sugar-cane juice. Some flavor, but very close to sugar water. From Taiwan. Which makes sense: In a Hong Kong store I saw cans of pure sugar water.

2. At Trader Joe’s I bought a package of trail mix called “Omega Trek Mix with Omega Fortified Cranberries.” (A new use of omega, by the way.) It contained “500 mg Omega-3 Fatty Acids Per Serving.” Sold only by Trader Joe’s. Not saying which omega-3 fats is a problem; so is lack of refrigeration. I could do a bio-assay, I realized: using the tests I have blogged about, such as balance and arithmetic, I could determine how much of the mix I had to eat to have the same effect as 1 tablespoon of flaxseed oil.

3. At Trader Joe’s I asked the checkout clerk what parts of her job she liked the best. “If we card a secret shopper, we get $15 for lunch,” she said. Lunch here? I asked. Lunch anywhere, she said. Whereas Dell employees detest secret shoppers. A tiny glimpse of a better future.

The Clouded Crystal Ball

“Does eating influence brain function?” begins a 1974 Scientific American article titled “Nutrition and the Brain” by 2 MIT professors. It is mainly about how changes in carbohydrate affect the brain — especially what happens after a carbohydrate-rich meal. A few studies of protein variation are discussed. Nothing about the effects of varying fat intake, although the brain is mostly fat.